40% Acrylamide Solution from Bio-Rad

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Item40% Acrylamide Solution
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1610140
Brand/TrademarkLSG LSD

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Hexanematic crossover in epithelial monolayers depends on cell adhesion and cell densityNature CommunicationsSeptember 16, 2023Julia Eckert, Benoît Ladoux, René-Marc Mège, Luca Giomi, Thomas Schmidtgel substrates Polyacrylamide (PAA) gels were prepared from a stock solution of 40% acrylamide: A (1610140, Bio Rad) and 2% bis-acrylamide: B (1610142, Bio Rad) in PBS. The ratio of these components were mixed

(2) Spatial transcriptomics for profiling the tropism of viral vectors in tissuesNature BiotechnologySeptember 1, 2023Min J. Jang, Gerard M. Coughlin, Cameron R. Jackson, Xinhong Chen, Miguel R. Chuapoco, Julia L. Vendemiatti, Alexander Z. Wang, Viviana Gradinaru(AM8439, Invitrogen), acrylic acid N-hydroxysuccinimide ester (AA-NHS, A8060, MilliporeSigma), acrylamide (1610140, Bio-Rad), bisacrylamide (1610142, Bio-Rad), tetramethylethylenediamine (TEMED, T7024, MilliporeSigma),

(3) Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structuresJournal of Biological ChemistryAugust 1, 2023Robert Bucki, Daniel V. Iwamoto, Xuechen Shi, Katherine E. Kerr, Fitzroy J. Byfield, Łukasz Suprewicz, Karol Skłodowski, Julian Sutaria, Paweł Misiak, Agnieszka Z. Wilczewska, et al.(G7776) were from Sigma-Aldrich. 2% (w/v) bis acrylamide (1610142) and 40% (w/v) acrylamide solutions (1610140) were from Bio-Rad Laboratories. TEMED (TB0508) was from Bio Basic. Ammonium persulfate (17-1311-01)

(4) Scalable projected Light Sheet Microscopy for high-resolution imaging of living and cleared samplesbioRxiv : the preprint server for biologyJuly 18, 2023Chen Y, Chauhan S, Gong C, Dayton H, Xu C, De La Cruz ED, Datta MS, Leong KW, Dietrich LEP, Tomer R.fixed brain samples were incubated in 1% hydrogel monomers (HMs) consisting of 1% w/v acrylamide (#1610140, BioRad), 0.05% w/v bisacrylamide (#1610142, BioRad), 4% paraformaldehyde (15710-S, Electron Microscopy

(5) The Effect of Substrate Stiffness on Elastic Force Transmission in the Epithelial Monolayers over Short TimescalesCellular and Molecular BioengineeringJuly 13, 2023Aapo Tervonen, Sanna Korpela, Soile Nymark, Jari Hyttinen, Teemu O. Ihalainengel rigidities were achieved by mixing different ratios of gel precursors acrylamide (AA, stock 40%, #1610140, Bio-Rad Laboratories, Hercules, USA) and bis-acrylamide (Bis, stock 2%, #1610142, Bio-Rad Laboratories,

(6) Long-range DNA end resection supports homologous recombination by checkpoint activation rather than extensive homology generationeLifeJune 30, 2023Michael T Kimble, Matthew J Johnson, Mattie R Nester, Lorraine S Symington,2% Bis Solution,Bio-Rad,Cat# 1610142, "chemical compound, drug",40% Acrylamide Solution,Bio-Rad,Cat# 1610140, "chemical compound, drug","N,N,N’,N’-Tetramethyl-ethylenediamine (TEMED)",Sigma,Cat# T9281-25mL, "chemical

(7) Nuclear lamina strain states revealed by intermolecular force biosensorNature CommunicationsJune 30, 2023Brooke E. Danielsson, Bobin George Abraham, Elina Mäntylä, Jolene I. Cabe, Carl R. Mayer, Anna Rekonen, Frans Ek, Daniel E. Conway, Teemu O. Ihalainennuclear volume. Hydrogel cushions were constructed from polyacrylamide (mixing ratio 12.5% acrylamide (#1610140, Bio-Rad Laboratories, Hercules, USA), 7.5% Bis-acrylamide (#1610142, Bio-Rad Laboratories, Hercules,

(8) Directionality of developing skeletal muscles is set by mechanical forcesNature CommunicationsMay 27, 2023Kazunori Sunadome, Alek G. Erickson, Delf Kah, Ben Fabry, Csaba Adori, Polina Kameneva, Louis Faure, Shigeaki Kanatani, Marketa Kaucka, Ivar Dehnisch Ellström, et al.kPa (0.5 kPa) gels, a 1000 μl stock solution containing HEPES 10 mM, 300 μl (75 μl) acrylamide 40% (1610140, Bio-Rad), 125 μl (30 μl) bisacrylamide 2% (1610142, Bio-Rad), 5 μl (5 μl) 10% ammonium persulfate diluted

(9) pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cellseLifeMay 22, 2023Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor CJ, Nelson CM, Toettcher JE.drug",Glutaraldehyde,Sigma-Aldrich,Cat # 340855, "Chemical compound, drug",40% acrylamide solution,Bio-Rad,Cat # 1610140, "Chemical compound, drug",2% bis-acrylamide solution,Bio-Rad,Cat # 161-0142, "Chemical compound,

(10) Matrix stiffness regulates tumor cell intravasation through expression and ESRP1-mediated alternative splicing of MENACell reportsApril 25, 2023Wang W, Taufalele PV, Millet M, Homsy K, Smart K, Berestesky ED, Schunk CT, Rowe MM, Bordeleau F, Reinhart-King CA.(PF573228),MilliporeSigma,PZ0117, ammonium persulfate (APS),Biorad,1610700EDU, Acrylamide [40% (w/v) solution],Biorad,1610140, bis-acrylamide [2% (w/v) solution],Biorad,1610142, CSF-1,R&D System,216-MC, Ribose,Thermo Fisher

(11) Matrix stiffness induces epithelial-to-mesenchymal transition via Piezo1-regulated calcium flux in prostate cancer cellsiScienceApril 21, 2023Lopez-Cavestany M, Hahn SB, Hope JM, Reckhorn NT, Greenlee JD, Schwager SC, VanderBurgh JA, Reinhart-King CA, King MR.bis-acrylamide.54 Gel precursor solutions were mixed for the 5kPa gel at a ratio of 7.5% (v/v) acrylamide solution (1610140, BIO RAD, Hercules, CA, USA) to 0.175% (v/v) bis-acrylamide solution (1610142, BIO RAD, Hercules, CA,

(12) Nuclear export inhibition jumbles epithelial-mesenchymal states and gives rise to migratory disorder in healthy epitheliaeLifeFebruary 21, 2023Krull CM, Li H, Pathak A.,Rhodamine Phalloidin,Invitrogen,Cat. #: R415,(1:250) "Chemical compound, drug",40% Acrylamide Solution,BIORAD,Cat. #:1610140,N/A "Chemical compound, drug",2% Bis Solution,BIORAD,Cat. #: 1610142,N/A "Chemical compound, drug",Bind

(13) Full-length huntingtin is palmitoylated at multiple sites and post-translationally myristoylated following caspase-cleavageFrontiers in PhysiologyJanuary 13, 2023Fanny L. Lemarié, Shaun S. Sanders, Yen Nguyen, Dale D. O. Martin, Michael R. Hayden"30% Acrylamide/Bis Solution, 29:1",Bio-Rad,#1610156 Western Blot Analysis,40% Acrylamide Solution,Bio-Rad,#1610140 List of reagents and chemicals used.

(14) Protein disulfide isomerase A3 activity promotes extracellular accumulation of proteins relevant to basal breast cancer outcomes in human MDA-MB-A231 breast cancer cellsAmerican Journal of Physiology-Cell PhysiologyJanuary 1, 2023Anna Germon, Kate J. Heesom, Reiss Amoah, Josephine C. AdamsCulture Plate,Falcon,353934 60-mm TC-treated cell culture dishes,Falcon,353002 Acrylamide (40%),Bio-Rad,1610140 Ammonium persulfate powder (APS),BDH (now VWR Life Science),BDH9214-500G 20- to 200-μL sterile filter

(15) A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistrySTAR ProtocolsDecember 16, 2022Zhengyuan Pang, Li YeInstruments,N/A "4′,6-diamidino-2-phenylindole (DAPI)",Sigma-Aldrich,Cat#D9542; CAS#28718-90-3 40% acrylamide solution,Bio-Rad,Cat#1610140 2% bis-acrylamide solution,Bio-Rad,Cat#1610142 32% PFA solution,Electron Microscopy Sciences,Cat#15714-S "Phosphate

(16) Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infectionCell reportsDecember 13, 2022Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM.were immersed in an A1P4 CLARITY solution (4% paraformaldehyde (EMS #15710), 1% acrylamide (Bio-Rad #1610140), 0.05% bis acrylamide (Bio-Rad #1610142), 0.0025 g/mL radical initiator (Wako Chemical #VA-044) in

(17) Light-sheet photonic force optical coherence elastography for high-throughput quantitative 3D micromechanical imagingNature CommunicationsDecember 1, 2022Yuechuan Lin, Nichaluk Leartprapun, Justin C. Luo, Steven G. Adieprepared as previously described37. Briefly, acrylamide monomer (Bio-Rad, 40% Acrylamide Solution, 1610140), bis-acrylamide crosslinker (Bio-Rad, 2% Bis Solution, 1610142), and deionized water were mixed at

(18) Cooperation between myofibril growth and costamere maturation in human cardiomyocytesFront Bioeng BiotechnolNovember 1, 2022Huaiyu Shi, Chenyan Wang, Bruce Z. Gao, James H. Henderson, Zhen Maet al., 2016), starting at the preparation of prepolymer solution, made of acrylamide (BIO-RAD Ca# 1610140), bis-acrylamide (BIO-RAD Ca# 1610142), 35 mM HEPES buffer (SIGMA Ca# 7365-45-9), 0.1% w/v ammonium

(19) Mechanical coupling of supracellular stress amplification and tissue fluidization during exit from quiescenceProceedings of the National Academy of SciencesAugust 9, 2022Emma Lång, Christian Pedersen, Anna Lång, Pernille Blicher, Arne Klungland, Andreas Carlson, Stig Ove BøeNext, polyacrylamide gels (4.0 kPa) were prepared by mixing a 93.75 µL 40% (7.5%) acrylamide solution (1610140; Bio-Rad), 37.5 µL 2% Bis-solution (161-0142; Bio-Rad), 2 µL (0.4%) carboxylate-modified fluorescent

(20) Involvement of Mechanical Cues in the Migration of Cajal-Retzius Cells in the Marginal Zone During Neocortical DevelopmentFrontiers in Cell and Developmental BiologyMay 16, 2022Ana López-Mengual et al.cells. PAA gels with different stiffness were generated by modifying the proportion of acrylamide 40% (1610140; BioRad) and Bis-acrylamide Solution (2% w/v; 10193523; ThermoFisher) and the level of crosslinking

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